Popular guidelines

What is c1 v1 c2 v2?

What is c1 v1 c2 v2?

The formula for calculating a dilution is (C1) (V1) = (C2) (V2) whereC1 is the concentration of the starting solution. V1 is the volume of the starting solution. C2 is the concentration of the final solution. V2 is the volume of the final solution.

What is a 1 to 2 dilution?

For example, a 1:2 serial dilution is made using a 1 mL volume of serum. This expression indicates that 1 mL of serum is added to 1 mL of H20 and then mixed. This initial dilution is 1:2. Then, 1 mL of this dilution is added to 1 mL of H20 further diluting the sample.

What is a 1 to 3 dilution?

a dilution of 1:3 (one to three) means mix one part concentrate with. three parts water. it doesn’t mean mix a 33% solution.

What does 2% dilution mean?

Answered Novem. a 1:2 dilution is usually used for Volume #1 out of Volume #2 . Vol1/vol2 . In this case you want a certain substance Volume. And double the amount of solvent to dilute it .

What is my dilution factor?

The dilution factor is the inverse. That is, it is the number of times you multiply the new concentration to get to the original concentration; equivalently, it’s the number of times more volume of solvent you add to a given volume of your stock. So the dilution factor between 1.2 microgram/mL and 1.8 mg/mL is 1500.

What is dilution rate?

The dilution rate is calculated by dividing the flow rate (how much media flows into the vessel per hour) by the culture volume. For example, using a volume of 300 ml a dilution rate of 0.1 means that 30 ml of media is added to the culture every hour.

What is critical dilution rate?

The highest possible dilution rate at which steady state is able to be attained within a constant volume *bioreactor…

What is a 1 1 dilution?

the dilution ratio is the ratio of solute to solvent. In the given 1:1 dilution we combining 1 unit volume of solute with 1 unit volume of solvent to give 2 total units of total volume and in 1:2 dilution we combine 1unit of solute with 1 unit of solvent to give 3 total units of total volume .

What are disadvantages of continuous culture?

Disadvantages include: 1 The control of the production of some non-growth related products is not easy. For this reason, the continuous process often requires feed-batch culturing and a continuous nutrient supply. 2 Wall growth and cell aggregation can also cause wash-out or prevent optimum steady-state growth.

What is the difference between batch culture and continuous culture?

The products remain in the fermenter until the completion of fermentation. Thus unlike batch fermentation, in continuous fermentation, the fermentation process never stops in between and it continues to run for a long period of time with the addition of nutrients and harvesting the metabolites at regular intervals.

What is continuous culture?

Continuous culture is an ‘open’-culture system for the cultivation of microorganisms or cells in which fresh sterilized medium is introduced at a steady flow rate and from which the culture fluid emerges at the same rate. Many types of continuous culture methods exist of which the most common is the chemostat.

Which growth phase is longer in continuous culture?

Exponential phase

What is lag growth phase?

Lag phase. The lag phase is an adaptation period, where the bacteria are adjusting to their new conditions. The length of the lag phase can vary considerably, based on how different the conditions are from the conditions that the bacteria came from, as well as the condition of the bacterial cells themselves.

What is batch and continuous culture?

• Fermentation is carried out by batch or continuous culture. Batch Culture. Fermentation is carried out in a closed fermenter, with nothing added or removed during the process (except venting of gas) Microorganisms and nutrients are left for a set period of time, during which the nutrient stock is depleted.